Bright Singh, I S; Cini, Achuthan; Rejish Kumar, V J; Manju, N J; Rosamma, Philip(Springer, November 28, 2008)
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Abstract:
A packed bed bioreactor (PBBR) was developed
for rapid establishment of nitrification in brackish
water hatchery systems in the tropics. The reactors were
activated by immobilizing ammonia-oxidizing (AMONPCU-
1) and nitrite-oxidizing (NIONPCU-1) bacterial
consortia on polystyrene and low-density polyethylene
beads, respectively. Fluorescence in situ hybridization
demonstrated the presence of autotrophic nitrifiers belong
to Nitrosococcus mobilis, lineage of b ammonia oxidizers
and nitrite oxidizer Nitrobacter sp. in the consortia. The
activated reactors upon integration to the hatchery system
resulted in significant ammonia removal (P\0.01) culminating
to its undetectable levels. Consequently, a
significantly higher percent survival of larvae was observed
in the larval production systems. With spent water the
reactors could establish nitrification with high percentage
removal of ammonia (78%), nitrite (79%) and BOD (56%)
within 7 days of initiation of the process. PBBR is configured
in such a way to minimize the energy requirements
for continuous operation by limiting the energy inputs to a
single stage pumping of water and aeration to the aeration
cells. The PBBR shall enable hatchery systems to operate
under closed recirculating mode and pave the way for
better water management in the aquaculture industry.
Description:
J Ind Microbiol Biotechnol (2009) 36:355–365
DOI 10.1007/s10295-008-0504-9
Bright Singh, I S; Kavitha, Ramachandran(December 3, 2003)
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Abstract:
The optimum growth requirements of two nitrifying consortia developed from treated sewage by enrichment technique
were determined by a series of experiments. There was total inhibition of nitrification at above 2.75 g r' NH/- Nand 2.5g r'
N02' - N and the ammonia oxidizing consortium preferred a pH at 8.5 and the nitrite oxidizing consortium a pH of 7.5 as the
optima for nitrification. Optimum temperatures were between 20° and 30°C for both the groups. As the rate of airnow was
increased from I to 7 Ilmin, the build-up of N02- -N increased 10-fold and the consumption of N02- -N increased by a factor
of 28.8 implying that the ammonia oxidizing consortium in a bioreactor required three times more aeration than that for
nitrite oxidizers for ex pressing their full nitrifying potential. These data directly contribute for developing a fermentati on
process for the mass production of nitrifiers as well as for designing bio reactors for nitrifying sewage.
Description:
Indian lournal of Experimental Biology
Vol. 42, March 2004, pp. 314-318
Bright Singh, I S; Rosamma, Philip; Rejish Kumar, V J; Cini, Achuthan; Manju, N J(Springer, September 21, 2008)
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Abstract:
For establishing nitrification in prawn (non-penaeid, salinity 10–15 ppt) and
shrimp (penaeid, salinity 30–35 ppt) larval production systems, a stringed bed suspended
bioreactor (SBSBR) was designed, fabricated, and validated. It was fabricated with 5 mm
polystyrene and low density polyethylene beads as the substrata for ammonia and nitrite
oxidizing bacterial consortia, respectively, with an overall surface area of 684 cm2. The
reactors were activated in a prototype activator and were transported in polythene bags to
the site of testing. Performance of the reactors activated with the nitrifying bacterial
consortia AMONPCU-1 (ammonia oxidizers for non-penaeid culture) and NIONPCU-1
(nitrite oxidizers for non-penaeid culture) was evaluated in a Macrobrachium rosenbergii
larval rearing system and those activated with AMOPCU-1 (ammonia oxidizers for penaeid
culture) and NIOPCU-1 (nitrite oxidizers for penaeid culture) in a Penaeus monodon
seed production system. Rapid setting up of nitrification could be observed in both the
static systems which resulted in a higher relative per cent survival of larvae
Description:
Aquacult Int (2009) 17:479–489
DOI 10.1007/s10499-008-9218-2