Description: | Department of Marine Biology, Microbiology and Biochemistry, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/2176 |
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Dyuthi-T0527.pdf | (2.543Mb) |
Abstract: | Aim: To develop a new medium for enhanced production of biomass of an aquaculture probiotic Pseudomonas MCCB 103 and its antagonistic phenazine compound, pyocyanin. Methods and Results: Carbon and nitrogen sources and growth factors, such as amino acids and vitamins, were screened initially in a mineral medium for the biomass and antagonistic compound of Pseudomonas MCCB 103. The selected ingredients were further optimized using a full-factorial central composite design of the response surface methodology. The medium optimized as per the model for biomass contained mannitol (20 g l)1), glycerol (20 g l)1), sodium chloride (5 g l)1), urea (3Æ3 g l)1) and mineral salts solution (20 ml l)1), and the one optimized for the antagonistic compound contained mannitol (2 g l)1), glycerol (20 g l)1), sodium chloride (5Æ1 g l)1), urea (3Æ6 g l)1) and mineral salts solution (20 ml l)1). Subsequently, the model was validated experimentally with a biomass increase by 19% and fivefold increase of the antagonistic compound. Conclusion: Significant increase in the biomass and antagonistic compound production could be obtained in the new media. Significance and Impact of the Study: Media formulation and optimization are the primary steps involved in bioprocess technology, an attempt not made so far in the production of aquaculture probiotics. |
URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2034 |
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Optimization of carbon and nitrogen....pdf | (662.4Kb) |
Description: | Fishing Technology Division, Central Institute of Fisheries Technology |
URI: | http://dyuthi.cusat.ac.in/purl/2148 |
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Dyuthi-T0507.pdf | (10.59Mb) |
Abstract: | We are in the cutting edge of a new era of development without leaving any promises to next generation. But the scale and size of the problem are only partially blamed. The juggernaut of Globalisation has trampled upon whatever little hope we might have had making a quick transition to a less energy – intensive world. “Environment friendliness begins at home”. Our quest for productivity and profitability should progress simultaneous with our cooperative responsibility of leaving behind a clean and green earth for the generation to come. Climate change is the most pressing global environmental challenge being faced by humanity, with the quest for better productivity for our fragile ecosystem. It is too late to rely solely on reduction in Green house gas emissions to mitigate climate change although this is undoubtedly crucial. Coastal belts are more prone to these devastating impacts and its protection is an intensive filed of research. The present study describes how the colourful Carotenoids and Chlorophylls can be used in rapid hand on tool in conjunction with molecular biology to open sources and it also explores the fate of organic matter in the aquatic system and underlying sediments. |
Description: | Department of Chemical Oceanography, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/2151 |
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Dyuthi-T0510.pdf | (7.907Mb) |
URI: | http://dyuthi.cusat.ac.in/purl/2130 |
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Dyuthi-T0490.pdf | (3.366Mb) |
Abstract: | The objective of present investigation was to study the population genetic structure of S. longiceps by applying three different basic population genetic techniques such as cytogenetics, non-enzymatic biochemicalgenetics (general protein) and morphomeristics/metrics. |
URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2090 |
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Dyuthi-T0442.pdf | (5.520Mb) |
Abstract: | The main source of protein for human and animal consumption is from the agricultural sector, where the production is vulnerable to diseases, fluctuations in climatic conditions and deteriorating hydrological conditions due to water pollution. Therefore Single Cell Protein (SCP) production has evolved as an excellent alternative. Among all sources of microbial protein, yeast has attained global acceptability and has been preferred for SCP production. The screening and evaluation of nutritional and other culture variables of microorganisms are very important in the development of a bioprocess for SCP production. The application of statistical experimental design in bioprocess development can result in improved product yields, reduced process variability, closer confirmation of the output response to target requirements and reduced development time and overall cost.The present work was undertaken to develop a bioprocess technology for the mass production of a marine yeast, Candida sp.S27. Yeasts isolated from the offshore waters of the South west coast of India and maintained in the Microbiology Laboratory were subjected to various tests for the selection of a potent strain for biomass production. The selected marine yeast was identified based on ITS sequencing. Biochemical/nutritional characterization of Candida sp.S27 was carried out. Using Response Surface Methodology (RSM) the process parameters (pH, temperature and salinity) were optimized. For mass production of yeast biomass, a chemically defined medium (Barnett and Ingram, 1955) and a crude medium (Molasses-Yeast extract) were optimized using RSM. Scale up of biomass production was done in a Bench top Fermenter using these two optimized media. Comparative efficacy of the defined and crude media were estimated besides nutritional evaluation of the biomass developed using these two optimized media. |
Description: | Dept. of Marine Biology, Microbiology and Biochemistry, Cochin University of Science and Technology, |
URI: | http://dyuthi.cusat.ac.in/purl/2363 |
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Dyuthi-T0635.pdf | (5.653Mb) |
Abstract: | This thesis presents a detailed account of a cost - effective approach towards enhanced production of alkaline protease at profitable levels using different fermentation designs employing cheap agro-industrial residues. It involves the optimisation of process parameters for the production of a thermostable alkaline protease by Vibrio sp. V26 under solid state, submerged and biphasic fermentations, production of the enzyme using cell immobilisation technology and the application of the crude enzyme on the deproteinisation of crustacean waste.The present investigation suggests an economic move towards Improved production of alkaline protease at gainful altitudes employing different fermentation designs utilising inexpensive agro-industrial residues. Moreover, the use of agro-industrial and other solid waste substrates for fermentation helps to provide a substitute in conserving the already dwindling global energy resources. Another alternative for accomplishing economically feasible production is by the use of immobilisation technique. This method avoids the wasteful expense of continually growing microorganisms. The high protease producing potential of the organism under study ascertains their exploitation in the utilisation and management of wastes. However, strain improvement studies for the production of high yielding variants using mutagens or by gene transfer are required before recommending them to Industries.Industries, all over the world, have made several attempts to exploit the microbial diversity of this planet. For sustainable development, it is essential to discover, develop and defend this natural prosperity. The Industrial development of any country is critically dependent on the intellectual and financial investment in this area. The need of the hour is to harness the beneficial uses of microbes for maximum utilisation of natural resources and technological yields. Owing to the multitude of applications in a variety of industrial sectors, there has always been an increasing demand for novel producers and resources of alkaline proteases as well as for innovative methods of production at a commercial altitude. This investigation forms a humble endeavour towards this perspective and bequeaths hope and inspiration for inventions to follow. |
URI: | http://dyuthi.cusat.ac.in/purl/2482 |
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Dyuthi-T0665.pdf | (11.57Mb) |
Abstract: | In Vembanad Lake and its confluent rivers (Kerala, India), the catches of Macrobrachium rosenbergii (de Man) were reported to have dwindled to a mere 39 t in the 1980s from average landings of 300 t during the 1960s. This decline is due to the impact of a number of human interventions affecting the ecosystem and, hence, the stocks of M. rosenbergii. Monitoring of landings in 1994-1995 and 1995-1996 indicates an improvement in catches. This paper discusses the reasons for the decline and revival in stocks and suggestions for their replenishment. |
URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2018 |
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Reviving the Macrobrachium rosenbergii....pdf | (271.5Kb) |
Abstract: | The gel strength, compressibility and folding characteristic of suwari (set) and kamaboko (set and cooked) gels prepared from rohu (Labeo rohita), catla (Catla catla) and mrigal (Cirrhinus mrigala) surimi were examined to understand the occurrence of suwari and modori phenomena in surimi from major freshwater carps. Suwari setting of gels did not take place at lower temperatures. Suwari gels showed good gel strength at 50 °C for rohu and at 60 °C for catla and mrigal after 30 min setting time. Incubation for 60 min decreased the gel strength at 60 °C for rohu and catla. Setting at 25 °C followed by cooking at 90 °C increased the gel strength. Increased setting temperature, however, decreased the gel strength of cooked gels. Gel strength and compressibility data were supported by folding characteristics. |
URI: | http://dyuthi.cusat.ac.in/purl/2512 |
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Dyuthi-AR01.pdf | (168.5Kb) |
Description: | Department of Chemical Oceanography,Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/2377 |
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Dyuthi-T0649.pdf | (16.69Mb) |
Abstract: | Humic substances are complex polymeric structures.No other polymers with such a wide range of properties are so widely distributed in nature.But still their moleculer structures are unknown. A structural knowledge is essential in determining their reactivity with metals.In the present work structural elucidation of humic acids from three different mangrove ecosystems of Cochin area is done with the available data from functional group analysis and various spectroscopic methods.13C NMR spectra of the solid samples with CPMAS,IR and SEM are very promising in revealing the complex structures of these polymeric substances.Sorptional studies on the sediment and humic acid of mangrove ecosystem reveals that the major portion of the organic matter is not extractable with Sodium hydroxide and humic acid only a small portion of the total organic matter. Humic acid is a good complexing agent and scavenger. Due to the nonextractable nature of the organic matter present with the sediment left after alkali extraction it is a better scavenger. |
Description: | Department of Chemical Oceanography,Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/2125 |
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Dyuthi-T0455.pdf | (3.568Mb) |
URI: | http://dyuthi.cusat.ac.in/purl/2198 |
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Dyuthi-T0549.pdf | (3.278Mb) |
Abstract: | The present study of the parasitic copepods gives the taxonomic description of thirty one species of parasites collected from various elasmobranch fishes of Kerala coast. Copepods parasitic on fishes include three sub orders, viz. poecilostomatoida, cyclopoida and siphonostomatoida. Parasitic copepods of elasmobranch fishes for the present study were collected from different fish landing centres of Kerala and by undertaking regular fishing trips. The collected parasites are identified to the species level and described. It is found that out of thirty one species, fifteen are new to science. They belong to the genera viz. Nothobomolochus Vervoot, 1962, Caligus Muller, 1785, Alebion, Kroyer, 1863, Gloipotes Steenstrup and Lutken, 1861, Pandarus Leach,1819, Perissopus Steenstrup and Lutken, 1861, Echthrogaleus Steenstrup and Lutken, 1861 and Kroyeria van Beneden, 1853. Fifteen new host records were reported. Two genera viz. Echthrogaleus and Entepherus were reported for first time from Indian waters. A new genus called Penicillus was erected. The general observations made during this study revealed certain interesting aspects of host-parasite relationship, host specificity, site specificity, adaptive modifications and geographical distribution. |
Description: | Department of Industrial Fisheries, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2092 |
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Dyuthi-T0459.pdf | (3.429Mb) |
Description: | Department of Physical Oceanography, School of Marine Sciences,Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/2202 |
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Dyuthi-T0553.pdf | (7.617Mb) |
URI: | http://dyuthi.cusat.ac.in/purl/2267 |
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Dyuthi-T0577.pdf | (6.850Mb) |
Abstract: | |
Description: | Department of Marine Biology, Microbiology and Biochemistry |
URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2085 |
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Dyuthi-T0540.pdf | (8.578Mb) |
Description: | Chemical Oceanography Division, School of Marine Sciences, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/purl/2178 |
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Dyuthi-T0528.pdf | (4.017Mb) |
Description: | Division of Marine Biology, Micrbiology and Biochemistry,Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2053 |
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Dyuthi-T0541.pdf | (9.131Mb) |
Description: | School of Marine Sciences, Cochin University of Science and Technology |
URI: | http://dyuthi.cusat.ac.in/xmlui/purl/2012 |
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Dyuthi-T0466.pdf | (7.310Mb) |
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