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| Abstract: | Process parameters influencing e-glutaminase production by marine Vibrio costicola in solid state fermentation (SSF) using polystyrene as an inert support were optimised. Maximal enzyme yield (157 U/g dry substrate) was obtained at 2% (w/w) t:glutamine, 35°C and pH 7.0 after 24 h. Maltose and potassium dihydrogen phosphate at 1% (w/w) concentration enhanced enzyme yield by 23 and 18%, respectively, while nitrogen sources had an inhibitory effect. Leachate with high specific activity for glutaminase (4.2 U/mg protein) and low viscosity (0-966 Ns/m 2) was recovered from the polystyrene SSF system |
| Description: | Process Biochemistry, Vol. 32, No. 4, pp. 285-289, 1997 |
| URI: | http://dyuthi.cusat.ac.in/purl/4243 |
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| Impact of proce ... ne as an inert support.pdf | (581.2Kb) |
| Abstract: | Use of inert supports have been recommended for SSF in on ar to overcome its inherent problems and efforts are being made to search for newer and better materials to act as inert solid supports lidoo et al, 1982; Zhu et al, 1994).In the present study an attempt is made to produce L-glutaminase, which is industrially and therapeutically impo rtant, from marine bacteria under solid state fermentation using natura.l. inert and mixed substrates with a view to develop an ideal bioprocess for its large scale production. |
| Description: | Microbial Technology Unit, Department of Biotechnology, Cochin University of Science and Technology |
| URI: | http://dyuthi.cusat.ac.in/purl/3370 |
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| Dyuthi-T1347.pdf | (6.136Mb) |
| Abstract: | L - Glutaminase, a therapeutically and industrially important enzyme, was produced from marine Vibrio costicola by a novel solid state fermentation process using polystyrene beads as inert support. The new fermentation system offered several advantages over the conventional systems, such as the yield of leachate with minimum viscosity and high specific activity for the target product besides facilitating the easy estimation of biomass. The enzyme thus produced was purified and characterised. It was active at physiological pH, showed high substrate specificity towards L - glutamine and had a Km value of 7.4 x 10-2 M. It also exhibited high salt and temperature tolerance indicating good scope for its industrial and therapeutic applications |
| Description: | Brazilian Archives of Biology and Technology 42(3),pp-363-368 |
| URI: | http://dyuthi.cusat.ac.in/purl/4266 |
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| Purification an ... e Fermentation Process.pdf | (31.31Kb) |
| Abstract: | Polystyrene beads, impregnated with mineral salts/glutamine medium as inert support, were used to produce L-glutaminase from Vibrio costicola by solid-state fermentation. Maximum enzyme yield, 88 U/g substrate, was after 36 h. Glucose at 10 g/kg enhanced the enzyme yield by 66%. The support system allowed glutaminase to be recovered with higher specific activity and lower viscosity than when a wheat-bran system was used |
| Description: | World Journal of Microbiology & Biotechnology 11,683-684 |
| URI: | http://dyuthi.cusat.ac.in/purl/4246 |
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| Polystyrene-an ... lld-state fermentation.pdf | (138.6Kb) |
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