Bright Singh, I S; Sahul Hameed, A S; Parameswaran, V; Ravi, Shukla; Thirunavukkarasu, A R; Bhonde, R R(Elsevier, January 8, 2006)
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Abstract:
A continuous cell line (SISK) from kidney of sea bass, Lates calcarifer, has been established and characterized. The cell line
was maintained in Leibovitz' L-15 supplemented with 15% fetal bovine serum. This cell line has been subcultured more than 100
times over a period of 2 years. The SISK cell line consists of predominantly of epithelial-like cells. These cells showed strong
positive for epithelial markers such as cytokeratin 19 and pancytokeratin. The cells were able to grow at temperature between 25
and 32 °C with optimum temperature of 28 °C. The growth rate of sea bass kidney cells increased as the FBS proportion increased
from 2% to 20% at 28 °C with optimum growth at the concentrations of 15% or 20% FBS. The distribution of chromosome number
was 30 to 56 with a modal peak at 48 chromosomes. Polymerase chain reaction products were obtained from SISK cells and tissues
of sea bass with primer sets of microsatellite markers of sea bass. Five fish viruses were tested on this cell line to determine its
susceptibility to these viruses and this was found to be susceptible to MABV NC1 and nodavirus, and the infection was confirmed
by RT-PCR and CPE. This suggests that the SISK cell line has good potential for the isolation of various fish viruses. This cell line
has been shown to be susceptible to bacterial extracellular products. The SISK cell line is the India's first marine fish cell line.
Gopinath, Prem; Dr.Bright Singh, I S(Cochin University of Science & Technology, March , 2014)
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Abstract:
The present study was initiated when several massive outbreaks of Chikungunya, Dengue and Japanese Encephalitis were frequently reported across the State of Kerala. Multiple symptoms persisted among the affected individuals and the public health officials were in search of aetiological agents responsible for the out breaks and, other than clinical samples no resources were available. In this context, a study was undertaken to focus on mosquito larvae to investigate the viruses borne by them which remain silently prevalent in the environment. The study was not a group specific investigation limited to either arbovirus or enterovirus, but had a broad spectrum approach. The study encompassed the viral pathogens that could be isolated, their impact when passaged through cell lines, growth kinetics, titer of the working stocks in specific cell line, the structure by means of transmission electron microscopy(TEM), the one step growth and molecular characterization using molecular tools.