Gopakumar, S D; Dr. Gopinathan, C P(Cochin University of Science & Technology, August , 2004)
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Abstract:
The present study is an attempt to standardize the environmental condition like pH, salinity and photoperiod, and also the feed for the maximum production of rotifers. Considering the deficiency of essential fatty acids in rotifers, enrichment experiments were carried out and fatty acids profile were analysed. Attempts were made to improve the production of clown fish (Amphiprion sebae) juveniles using enriched rotifers. Attempts were also made to rear various larval stages of Penaeus monodon with enriched rotifers as a substitute for Artemia nauplii.
Bright Singh, I S; Swapna, Antony P; Sudheer, N S; Vrinda, S; Priyaja, P; Rosamma, Philip(Elsevier, May 20, 2010)
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Abstract:
A crustinlike
antimicrobial peptide from the haemocytes of giant tiger shrimp, Penaeus monodon was
partially characterized at the molecular level and phylogenetic analysis was performed. The partial coding
sequence of 299 bp and 91 deduced amino acid residues possessed conserved cysteine residues characteristic
of the shrimp crustins. Phylogenetic tree and sequence comparison clearly confirmed divergence
of this crustinlike
AMP from other shrimp crustins. The differential expression of the crustinlike
AMP
in P. monodon in response to the administration of various immunostimulants viz., two marine yeasts
(Candida haemulonii S27 and Candida sake S165) and two bglucan
isolates (extracted from C. haemulonii
S27 and C. sake S165) were noted during the study. Responses to the application of two grampositive
probiotic bacteria (Bacillus MCCB101 and Micrococcus MCCB104) were also observed. The immune profile
was recorded preand
postchallenge
white spot syndrome virus (WSSV) by semiquantitative
RTPCR.
Expressions of seven WSSV genes were also observed for studying the intensity of viral infection in
the experimental animals. The crustinlike
AMP was found to be constitutively expressed in the animal
and a significant downregulation
could be noted postchallenge
WSSV. Remarkable downregulation
of the gene was observed in the immunostimulant fed animals prechallenge
followed by a significant
upregulation
postchallenge
WSSV. Tissuewise
expression of crustinlike
AMP on administration of C.
haemulonii and Bacillus showed maximum transcripts in gill and intestine. The marine yeast, C. haemulonii
and the probiotic bacteria, Bacillus were found to enhance the production of crustinlike
AMP and
confer significant protection to P. monodon against WSSV infection
Prabhakaran, N; Dr. Sivadas, P(Cochin University of Science and Technology, April 30, 1990)
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Abstract:
Man's concern with environmental deterioration is one of the major reasons for the increased interest in marine and estuarine microbes. Microbes form an important link in the biogeochemical cycling and their cyclinq activites often determine to a large measure the potential productivity of an ecosystem In the recycling of the nutrients in the estuary,
bacteria and fungi therefore play a particularly significant role.The allochthonous plant materials contain biopolymers such as cellulose, lignin, humus etc., that are difficult to degrade into simpler substances. The fungi have the ability to degrade _substances, thereby making them available for cycling within the system. The present study is devoted to find the composition and the activity of myco populations of Cochin backwater. For convenience the thesis is divided into eight chapters. The opening chapter briefly reviews the literature and projects the importance of work and the main objectives. Second chapter discusses the materials and methods. In the third chapter the systematic and taxonomy of estuarine yeasts are examined in detail since this information is scarcely available for our waters. The general ecological aspects of the yeasts and filamentous fungi in the area of study are examined in the fourth chapter using appropriate statistical techniques. A special reference to the fungi in a small mangrove ecosystem is attempted in the fifth chapter. The biochemical studies are discussed in the sixth chapter and the penultimate chapter provides an overall discussion. In the last chapter the summary of the work is presented.