Sherin, Antony; Dr. Paulose, C S(Cochin University of Science & Technology, May , 2010)
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Abstract:
In the present study, a detailed investigation on the alterations of
muscarinic M1, M3, α7 nicotinic acetylcholine receptor (α7 nAchR), GABA
receptors and its subtypes; GABAAα1 and GABAB in the brain regions of
streptozotocin induced diabetic and insulin induced hypoglycemic rats were
carried out. Gene expression of acetylcholine esterase (AChE), choline
acetyltransferase (ChAT), GAD, GLUT3, Insulin receptor, superoxide dismutase
(SOD), Bax protein, Phospholipase C and CREB in hypoglycemic and
hyperglycemic rat brain were studied. Muscarinic M1, M3 receptors, AChE,
ChAT, GABAAα1, GABAB, GAD, Insulin receptor, SOD, Bax protein and
Phospholipase C expression in pancreas was also carried out. The molecular
studies on the CNS and PNS damage will elucidate the therapeutic role in the
corrective measures of the damage to the brain during hypoglycemia and
hyperglycemia.
Description:
Department of Biotechnology,Cochin University of Science and Technology
Gireesh,G; Dr. Paulose, C S(Cochin University of Science & Technology, January , 2007)
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Abstract:
The present work is to understand the alterations of total Muscarinic and
Muscarinic MI receptors in brain and pancreatic islets of Streptozotocin induced
diabetic rats. The work focuses on the evaluation of the antihyperglycemic activity
of aqueous extracts of Aegle marmelose and Costus pictus leaves in vivo and the
changes in the total Muscarinic and Muscarinic MI receptors during diabetes and
after the treatment with insulin. The insulin secretory activity of Aegle marmelose
and Costus pictus leaf extracts and the effect of cholinergic receptor agonist were
investigated in vitro using rat primary pancreatic islet culture. Muscarinic MI
receptor kinetics and gene expression during diabetes and regulation of insulin
secretion by Aegle marmelose and Costus pie/us leaf extracts will help us to
elucidate the role of Muscarinic and Muscarinic MI receptors in hyperglycemia
and the regulatory activity of these plant extracts on insulin secretion through
Muscarinic receptors.
Description:
Department of
Biotechnology, Cochin University of Science and Technology