Hatha, A A M; Vivekanandhan, A A; Julie Joice, G; Christol(Elsevier, May 28, 2004)
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Abstract:
Motile aeromonads isolated from the intestines of farm-raised freshwater fish such as Catla catla, Labeo rohita and
Ctenopharyngodon idella have been characterized to species level. Morphological and physiological grouping revealed 61%
Aeromonas hydrophila, 30% Aeromonas caviae, 7% Aeromonas sobria and 2% which remained unidentified. Hemolytic
activity was detected mostly in A. hydrophila, while only half of the A. sobria and A. caviae showed this activity. Antibiotic
resistance patterns of the strains revealed that they had acquired a relatively higher resistance to oxytetracycline, amoxycillin,
ampicillin, novobiocin and polymixin-B, implicating possible use of these antibiotics in the aquaculture systems.
Description:
International Journal of Food Microbiology 98 (2005) 131– 134
Bright Singh, I S; Seena, Jose; Rosamma, Philip; Mohandas, A; Jayesh, P(Elsevier, December 26, 2010)
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Abstract:
Lack of shrimp cell lines has hindered the study of pollutants which adversely affects shrimp health and
its export value. In this context a primary haemocyte culture developed from Penaeus monodon was
employed for assessing the cytotoxicity and genotoxicity of two heavy metal compounds, cadmium
chloride and mercuric chloride and two organophosphate insecticides, malathion and monocrotophos.
Using MTT assay 12 h IC50 values calculated were 31.09 16.27 mM and 5.52 1.16 mM for cadmium
chloride and mercuric chloride and 59.94 52.30 mg l 1 and 186.76 77.00 mg l 1 for malathion and
monocrotophos respectively. Employing Comet assay, DNA damage inflicted by these pollutants on
haemocytes were evaluated and the pollutants induced DNA damage in >60% of the cells. The study
suggested that haemocyte culture could be used as a tool for quantifying cytotoxicity and genotoxicity of
aquaculture drugs, management chemicals and pollutants
Budidaya menghasilkan sejumlah besar limbah, terdiri dari metabolisme oleh-produk, makanan sisa, materi
feses dan residu dari masukan profilaksis dan terapeutik, mengarah pada penurunan kualitas air dan wabah
penyakit. Bioremediasi, aplikasi mikroba / enzim untuk tambak, adalah metode yang sedang digunakan untuk meningkatkan kualitas air dan menjaga kesehatan
dan stabilitas sistem akuakultur. Bioremediasi melibatkan mineralisasi bahan organik menjadi karbon
dioksida, memaksimalkan produktivitas primer yang merangsang produksi udang, nitrifikasi dan denitrifikasi
untuk (1) menghilangkan kelebihan nitrogen dari tambak dan (2) mempertahankan beragam dan stabil
masyarakat kolam dimana patogen dikeluarkan dari sistem dan spesies diinginkan mendapatkan didirikan.
Selain dari bahan organik merendahkan (detritivorous) bakteri heterotrofik, nitrifikasi, bakteri denitrifikasi dan
fotosintesis umumnya digunakan dalam bioremediasi.
Rosamma, Philip; Preetha, R; Jayaprakash, N S; Bright Singh, I S(The Society for Applied Microbiology, 2007)
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Abstract:
Aim: To develop a new medium for enhanced production of biomass of an
aquaculture probiotic Pseudomonas MCCB 103 and its antagonistic phenazine
compound, pyocyanin.
Methods and Results: Carbon and nitrogen sources and growth factors, such as
amino acids and vitamins, were screened initially in a mineral medium for the
biomass and antagonistic compound of Pseudomonas MCCB 103. The selected
ingredients were further optimized using a full-factorial central composite
design of the response surface methodology. The medium optimized as per the
model for biomass contained mannitol (20 g l)1), glycerol (20 g l)1), sodium
chloride (5 g l)1), urea (3Æ3 g l)1) and mineral salts solution (20 ml l)1), and
the one optimized for the antagonistic compound contained mannitol (2 g l)1),
glycerol (20 g l)1), sodium chloride (5Æ1 g l)1), urea (3Æ6 g l)1) and mineral
salts solution (20 ml l)1). Subsequently, the model was validated experimentally
with a biomass increase by 19% and fivefold increase of the antagonistic
compound.
Conclusion: Significant increase in the biomass and antagonistic compound
production could be obtained in the new media.
Significance and Impact of the Study: Media formulation and optimization are
the primary steps involved in bioprocess technology, an attempt not made so
far in the production of aquaculture probiotics.
Bright Singh, I S; Preetha, R; Jayaprakash, N S(Inter-Research, March , 2007)
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Abstract:
Synechocystis MCCB 114 and 115 were segregated as putative probionts for shrimp
larvae from a collection of 54 cyanobacterial cultures enriched from seawater. On feeding Penaeus
monodon post-larvae with the cyanobacteria, the generic diversity of the intestinal bacterial flora
could be enhanced with substantial reduction or total absence of Vibrio spp. A significant difference
(p < 0.001) in the percent survival of batches of post-larvae fed on the cyanobacterial cultures was
observed and, on repeated challenge with V. harveyi, the relative percent survival of those batches
of larvae fed on Synechocystis MCCB 114 and 115 was significantly higher. The Synechocystis
MCCB 114 and 115 cultures were found to contain high levels of protein (34 to 43%), in addition to
carotenoids