Hatha, A A M; Shubhankar Ghosh, A; Selvam, D G; Neethu, C S; Saramma, A V(Marine Biological Association of India, January 15, 2014)
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Abstract:
Emergence of antibiotic resistance among aquaculture
pathogens has made it necessary to look into environment
friendly, effective and sustainable methods such as probiotic
and immunostimulants among others.. In the present study,
LAB were isolated from the gut of fish species namely Rastrelliger
kanagurta and analyzed for their antibacterial activity against
various fish, shrimp and human pathogens. Different LAB
species such as Lactobacillus plantarum, L. bulgaricus, L. brevis
and L. viridiscens were encountered in the gut of R. kanagurta.
Several strains showed good activity against fish, shrimp and
human pathogens. LAB from the gut of such marine species
may be developed as possible probiont for environment friendly
health management of fresh water, estuarine and marine
species currently exploited in aquaculture
Description:
J. Mar. Biol. Ass. India, 55 (2), 22-27, July-December 2013
Bright Singh, I S; Jayaprakash, N S; Preetha, R; PhIlip, R(The Society for Applied Microbiology, July 20, 2006)
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Abstract:
Aim: To develop a new medium for enhanced production of biomass of an
aquaculture probiotic Pseudomonas MCCB 103 and its antagonistic phenazine
compound, pyocyanin.
Methods and Results: Carbon and nitrogen sources and growth factors, such as
amino acids and vitamins, were screened initially in a mineral medium for the
biomass and antagonistic compound of Pseudomonas MCCB 103. The selected
ingredients were further optimized using a full-factorial central composite
design of the response surface methodology. The medium optimized as per the
model for biomass contained mannitol (20 g l)1), glycerol (20 g l)1), sodium
chloride (5 g l)1), urea (3Æ3 g l)1) and mineral salts solution (20 ml l)1), and
the one optimized for the antagonistic compound contained mannitol (2 g l)1),
glycerol (20 g l)1), sodium chloride (5Æ1 g l)1), urea (3Æ6 g l)1) and mineral
salts solution (20 ml l)1). Subsequently, the model was validated experimentally
with a biomass increase by 19% and fivefold increase of the antagonistic
compound.
Conclusion: Significant increase in the biomass and antagonistic compound
production could be obtained in the new media.
Significance and Impact of the Study: Media formulation and optimization are
the primary steps involved in bioprocess technology, an attempt not made so
far in the production of aquaculture probiotics
Description:
Journal of Applied Microbiology 102 (2007) 1043–1051