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Abstract:
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This thesis covers various aspects of viral diseases affecting shrimp aquaculture. The
research component of this thesis can be divided into four areas. The areas covered are: I) A
study to determine the prevalence of WSSV among the crustaceans in the Vembanad estuary, the
shrimp aquaculture farms surrounding the estuary, and the sea off Cochin coast, India using two
, sets of nested PCR primers. 2) An investigation to compare the sequence of six major structural
proteins of WSSV; vp28, vp26, vp 19, vp68, vp281, vp466 from different geographical locations
with that of an isolate from India. 3) Simultaneous occurrence of HPV, IHHNV, MBV and
WSSV in postlarvae of P. monodon from hatcheries in India was monitored by Polymerase
Chain Reaction. 4) A real time PCR procedure was developed for the quantitative analysis of
WSSV infection. The viral load of postlarvae from hatcheries in Kerala meant for aquaculture
was also determined using the quantitative PCR. |