Bright Singh, I S; Jose, Seena; Jayesh, P(Springer, August 14, 2012)
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Abstract:
Development of continuous shrimp cell lines
for effective investigation on shrimp viruses remains elusive
with an arduous history of over 25 years. Despite
presenting challenges to researchers in developing a cell
line, the billion dollar aquaculture industry is under viral
threat. Advances in molecular biology and various gene
transfer technologies for immortalization of cells have
resulted in the development of hundreds of cell lines from
insects and mammals, but yet not a single cell line has been
developed from shrimp and other marine invertebrates.
Though improved growth and longevity of shrimp cells
in vitro could be achieved by using modified growth media
this did not make any leap to spontaneous transformation;
probably due to the fact that shrimp cells inhibited neoplastic
transformations. Oncogenic induction and immortalization
are considered as the possible ways, and an
exclusive medium for shrimp cell culture and an appropriate
mode of transformation are crucial. In this review
status of shrimp cell line development and its future orientation
are discussed
Description:
Indian J. Virol. (July–September 2012) 23(2):244–251
DOI 10.1007/s13337-012-0089-9