An inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi

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An inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi

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dc.contributor.author Bright Singh, I S
dc.contributor.author Rosamma, Philip
dc.contributor.author Jayaprakash, N S
dc.contributor.author Preetha, R
dc.contributor.author Sreedharan, Prathapan
dc.contributor.author Seena, Jose
dc.contributor.author Vijayan, K K
dc.date.accessioned 2014-07-16T05:09:16Z
dc.date.available 2014-07-16T05:09:16Z
dc.date.issued 2010-09-01
dc.identifier.uri http://dyuthi.cusat.ac.in/purl/4047
dc.description Aquaculture Research, 2010, 41, 1452^1461 doi:10.1111/j.1365-2109.2009.02436.x en_US
dc.description.abstract Persistence of the antivibrio property of the potential antagonistic probiotics, Pseudomonas MCCB 102 and 103, at di¡erent temperatures, pH and in organic solvents was studied. The antivibrio compound was extracted, puri¢ed and characterized using thin-layer chromatography, high-pressure liquid chromatography, liquid chromatography-mass spectroscopy, UV^ Vis and nuclear magnetic resonance spectroscopy and identi¢ed as N-methyl-1-hydroxyphenazine, a phenazine antibiotic. The toxicity of the compound was tested in Penaeus monodon haemocyte culture and the IC50 valuewas found to be1.4 0.31mg L 1. The compound was found to be bacteriostatic at 0.5mg L 1. Its stability to varying temperature, pH, organic solvents, prolonged shelf-life and vibriostatic nature point to its suitability for prophylatic aquaculture application. en_US
dc.description.sponsorship Cochin University of Science and Technology en_US
dc.language.iso en en_US
dc.publisher Blackwell Publishing en_US
dc.subject antivibrio compound en_US
dc.subject N-methyl-1- hydroxyphenazine en_US
dc.subject liquid chromatography-mass spectroscopy en_US
dc.subject nuclear magnetic resonance spectroscopy en_US
dc.subject haemocyte culture en_US
dc.subject aquaculture en_US
dc.title An inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi en_US
dc.type Article en_US


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