Chandrasekaran, M; Nagendra, Prabhu G(Elsevier, 1997)
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Abstract:
Process parameters influencing e-glutaminase production by marine Vibrio costicola in solid state fermentation
(SSF) using polystyrene as an inert support were optimised. Maximal enzyme yield (157 U/g dry
substrate) was obtained at 2% (w/w) t:glutamine, 35°C and pH 7.0 after 24 h. Maltose and potassium
dihydrogen phosphate at 1% (w/w) concentration enhanced enzyme yield by 23 and 18%, respectively, while
nitrogen sources had an inhibitory effect. Leachate with high specific activity for glutaminase (4.2 U/mg
protein) and low viscosity (0-966 Ns/m 2) was recovered from the polystyrene SSF system
Description:
Process Biochemistry, Vol. 32, No. 4, pp. 285-289, 1997